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The proteins were extracted with 1.2 ml NETT solution (1% Triton X-100, 2.5 M NaCl, 0.9 M TrisHCl [pH 7.5], 0.25 M EDTA, 100 mM PMSF), 200 L lysate was used as input whilst the remaining 1000 L samples were incubated with 30 L HA-tagged or Flag-tagged magnetic beads at 4 C overnight
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In animal models, pre-treatment of neurons has shown a decrease in neuronal cell death caused by glutamate excitotoxicity as well [65]
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