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In humans, the biotin-requiring enzymes include acetyl-CoA carboxylase (ACC: humans express two distinct ACC genes), pyruvate carboxylase (PC), propionyl-CoA carboxylase (PCC), and 3-methylcrotonyl-CoA carboxylase (3MCC)

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This retention advantage may explain why some patients feel more prolonged effects from methylcobalamin even if blood levels look similar

That means we must get these essential fats from our diet or supplements