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Cryo-sections were permeabilized, blocked with DAKO containing 0.1% saponin for 1 h, and then stained with Phalloidin antibody (Abcam, 1:1000)

Cell culture Human promyelocytic (HL60) cells from ATCC were maintained in RPMI 1640 media containing 10% foetal bovine serum and 200U/ml penicillin and streptomycin at 37 0 C in a humidified atmosphere of 5% CO 2 and 95% air

Studies document effects on 31.2% of human genes (approximately 4,000+ genes) with 50% expression change, suppressing RNA production in 70% of 54 genes overexpressed in metastatic colon cancer

systemically examined the expression of GPxs in breast cancer cell lines and found that GPx5 and GPx7 were down-regulated in the human breast cancer MCF-7 and MDA-MB-231 cell lines and GPx6 was also down-regulated in MDA-MB-231cells compared with healthy breast MCF-10A cells [19]