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Antibiotics often reduce TMA production temporarily but the microbiome rebounds and the effect can be transient

If you started 10 weeks ago on Wegovy, you should be at 1.0 mg (weeks 912)

After washing with 1x PTW (1x PBS and 0.1% Tween 20), add DAPI (100 ng/mL) and incubate the fixed gonads for 10 min at room temperature, following by washing them with 1x PTW three times