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Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively
An Emerging Option: GLP-1 Receptor Agonists One of the newer areas of interest in MCAS research involves GLP-1 receptor agonists (GLP-1RAs), medications originally developed for diabetes and weight management
Tier 1: recommended for regular use Polyethylene glycol (MiraLAX)
Incompatible with strong oxidizing agents
