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For Caspase-3 staining, the tissues were blocked with 10% normal horse serum for 30 min then incubated with Caspase-3 antibody (1:300, BD Biosciences, Macquarie Park, NSW, Australia) overnight, followed by secondary antibodies (Goat anti-rabbit HRP, 1:200, Vector Laboratories, Burlingame, CA, USA) for 45 min
An ongoing study is evaluating the pharmacokinetics, safety, and dose optimization, but it is likely to be administered orally once-daily
Mike Haney: And that really focuses on the sort of outcome, right
doi:10.3389/fendo.2018.00672 Gabr RE, El-Sharkawy AMM, Schr M, et al
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