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Use orthogonal validation: Consider genetic NNMT knockdown or comparison with structurally distinct NNMT inhibitors when mechanistic attribution is central to the study
In dietinduced obese mice, NNMTi treatment significantly reduced liver weight, liver volume, and hepatic triglyceride levels, alleviated hepatic steatosis and inflammatory infiltration, downregulated inflammatory factors (such as TNF and IL6) and lipogenic genes (such as FAS), and upregulated lipolytic genes (such as ATGL) in the liver
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