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Add bacteriostatic water slowly down the side of the vial, never directly onto the lyophilized cake, and swirl gently rather than shaking

Three different regions of interest of size 6464pixels per image and a moving average of 10 was applied to avoid any artefacts due to cellular motion or very slow-moving particles

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Thyroid conditions: If youre taking levothyroxine, have active thyroid disease, or have a family history of medullary thyroid cancer, it is important to know that both medications carry a theoretical risk warning around thyroid tumours (based on rodent data, not confirmed in humans)