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Energies were reported every 1000 steps, with trajectory coordinates saved every 1000 steps in both DCD and PDB formats
Con: Some people are hesitant to use a suppository at all, due to embarrassment or simply the awkwardness of using them
Absorbing the upper plasma into another tube add with same volume Reagent I
The aortas were then incubated in freshly prepared SA--Gal staining solution at pH 6.0 overnight at 37 C, according to the manufacturers protocol
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