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Western blot Equal amounts (50 g) of RINm5F, INS-1, and MIN6 cell lysate were analyzed by Western blotting with rabbit anti-AMPK monoclonal antibody#ab32047 (Epitomics Technology, Berlingame, CA), rabbit anti-phospho-AMPK(Thr172) monoclonal antibody #2531 (Cell Signaling Technology, Beverly, MA), rabbit anti-PARP polyclonal antibody#9542S, anti-cleaved caspase-3, phospho-CREB/CREB, PDX1, phospho Akt/Akt and GAPDH (Cell Signaling Technology, Beverly, MA), respectively

GLP-1 agonist medications have been on the market since 2005 with the release of exenatide (Byetta, AstraZeneca)

This imbalance can disrupt the HPG axis short for the hypothalamic-pituitary-gonadal axis which links the brain and testes to regulate testosterone levels and sperm production