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Briefly, cell or tissue lysates were prepared using radioimmunoprecipitation assay (RIPA) buffer (comprising 50 mM Tris-HCl at pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, and 0.1% SDS) supplemented with a protease inhibitor cocktail (Roche)
Given the enhanced efficacy of the 5FU + metformin combination in AKP organoid models in 3D (Fig
Elimination of the internal food noise that previously occupied her thoughts
Planning for this is practical, not vain
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