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are supported by the Italian Ministry of HealthRicerca Corrente to IRCCS MultiMedica

Western blot analysis Protein lysates were obtained by homogenizing tissues or cell pellets in sample buffer containing 62.5 mM Tris-HCl (pH 6.8), 4% SDS, 200 mM dithiothreitol (DTT), 10% glycerol, and 0.001% bromophenol blue using a tissue to buffer ratio of 1:10 (w/v), followed by boiling

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As you move through this process, listen to your body and pay attention to your symptoms