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Autofluorescence excited at 405 nm was collected from 430 to 480 nm and values were used to subtract autofluorescence bleeding into the roGFP channel as described previously (Schwarzlnder et al., Ratiometric image analysis Images were imported into a custom written MatLab (The MathWorks, www.mathworks.de) analysis suite (M.D
Ferreira JP, Sharma A, Butler J, et al
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