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The amino acid or amino acid analogs used in the sequential coupling of step 2) are Fmoc protected amino acids, wherein Boc-His(Trt)-OH or Boc-His(Boc)-OH.DCHA is used for H-His 7 , Fmoc-Lys(Alloc)-OH is used for Lys 26 , and octadecanedioic acid mono-tert-butyl ester is used for the coupling of Octadecanedioic Acid
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