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Top 10 MS2 scans were performed in the ion trap with CID fragmentation (isolation window 0.5 Da

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General function: Involved in methyltransferase activity Specific function: Required for the methylation step in diphthamide biosynthesis Gene Name: DPH5 Uniprot ID: P32469 Molecular weight: 33847.0 Reactions General function: Involved in methyltransferase activity Specific function: Siroheme synthase involved in methionine biosynthesis Gene Name: MET1 Uniprot ID: P36150 Molecular weight: 66124.70313 Reactions General function: Involved in methyltransferase activity Specific function: Non-specific O-methyltransferase that catalyzes the 2 O- methylation steps in the ubiquinone biosynthetic pathway Gene Name: COQ3 Uniprot ID: P27680 Molecular weight: 36330.60156 Reactions General function: Involved in catalytic activity Specific function: Dethiobiotin + sulfur + 2 S-adenosyl-L- methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine Gene Name: BIO2 Uniprot ID: P32451 Molecular weight: 41883.80078 Reactions General function: Involved in transaminase activity Specific function: Catalyzes the transfer of the alpha-amino group from S- adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA)

DOI: 10.1016/j.brainres.2005.03.031 27