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A serious problem of MS in the identification of metabolites is ion suppression, which is a kind of matrix effect and is mainly caused by the different volatility of metabolites, due to their different nature and structure, which in turn affects the output of the number of ions in the gas phase reaching the detector and eventually leads to errors in the quantification of the analysis (Annesley, 2003

The combined stiffening of the ventricular and vascular systems, including both systemic and pulmonary circulations, significantly contributes to the pathophysiology of HFpEF [20, 21]