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To minimize discomfort, ensure the area is clean and relaxed, use a new needle, and inject slowly
The aerobic Fe-loaded C408A sample prepared for LC-MS/MS analysis was not treated with -(4-hydroxyphenyl)ethyl iodoacetamide (HPE-IAM) because the S -sulfonate adduct can only be detected in the absence of HPE-IAM and when trypsin digestion is limited to 2 h
hydrogen peroxide (H 2 O 2 ), hydroxyl (OH ) radicals, superoxide (O 2 ) anions, and nitric oxide (NO) are all examples of reactive oxygen species (Trachootham et al., 2008)
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