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GH is a counter-regulatory hormone to insulin, so prolonged use at higher doses can impair glucose tolerance

Or, if youre injecting subcutaneously, insert it into the skin at a 45 degree angle

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Because the CellTox Green Dye can be added in a no-step format by adding the dye directly to cells at plating, the CellTox Green Cytotoxicity Assay can be performed prior to the GSH/GSSG-Glo Assay without changes to either protocol