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65 ) (Addgene, 52961) for CRISPRCas9 KO or into pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro 66 (Addgene, 71236) for CRISPRi knockdown, as previously described 41
Avoid vigorous agitation, which may compromise peptide integrity
Mammalian proapoptotic factor ChaC1 and its homologues function as glutamyl cyclotransferases acting specifically on glutathione
