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A new cytotoxic, DNA interstrand crosslinking agent, 5-(Aziridin-1-YL)-4-hydroxylamino-2-nitrobenzamide, is formed from 5-(Aziridin-1-YL)-2,4-dinitrobenzamide (CB 1954) by a nitroreductase enzyme in Walker carcinoma cells

1 H NMR (300 MHz, DMSO) 8.23 (d, J = 7.4 Hz, 1H), 8.12 (d, J = 8.2 Hz, 1H), 7.41 (d, J = 6.6 Hz, 9H), 7.23 (s, 1H), 7.12 7.04 (m, 6H), 6.78 (d, J = 5.4 Hz, 1H), 6.67 (d, J = 11.0 Hz, 1H), 4.56 (d, J = 6.6 Hz, 1H), 4.14 (q, J = 7.4 Hz, 1H), 3.60 (s, 3H), 3.51 (d, J = 8.0 Hz, 2H), 2.78 (dq, J = 32.2, 7.5 Hz, 4H), 1.36 (d, J = 2.4 Hz, 24H)

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Tout organisme vivant en arobie gnre des radicaux libres, puisque toutes les ractions chimiques utilisent l'oxygne, molcule oxydante par excellence