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Ghrelin is the primary orexigenic hormone
After denaturation in Laemmli buffer (Tris 40 mM, DTT 100 mM, sodium dodecyl sulfate 2% (v/v), glycerol 5% (v/v), bromophenol blue 0.075% (m/v), pH 6.8), proteins were separated on a 4-12% Criterion XT Precast Gel (Bio-Rad) in XT MOPS buffer (Bio-Rad) at 120 V for 2 h
Split dosing protocols Some protocols divide the daily dose into morning and evening administrations
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