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If a protocol requires eight equal aliquots from one vial: 80 mg 8 = 10 mg total peptide per aliquot At 5 mL reconstitution (16 mg/mL): 10 mg 16 mg/mL = 0.625 mL , or 62.5 units At 8 mL reconstitution (10 mg/mL): 10 mg 10 mg/mL = 1.0 mL , or 100 units a full syringe The 8 mL figure gives a cleaner draw

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Eng J, Kleinman WA, Singh L, Singh G, Raufman JP (1992) Isolation and characterization of exendin-4, an exendin-3 analogue, from Heloderma suspectum venom