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Be clear: I am not advocating its use and since it is not approved for human use - its WADA banned - just exploring the philosophy of what should be banned
Temperature fluctuations, repeated freeze-thaw cycles, prolonged room-temperature exposure, and excessive light can all reduce peptide stability over time
Global protein abundance profiling by mass spectrometry Sample preparation (A) Flash frozen liver sections (~25 mg) from WT or Gclc / mice (3 biological replicates each per TMT sample) were thawed on ice and resuspended in DPBS, supplemented with protease (cOmplete, EDTA-free protease inhibitor cocktail, Roche, #11873580001) and phosphatase (PhosSTOP, Roche, #4906845001) inhibitor tablets
After 14 days, the mice were sacrificed, and their major organs and tissues were analyzed with a hematoxylin and eosin (H&E) stain (Supplementary Fig
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