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After 48 h, biofilms were washed once with 1 PBS followed by incubation for 24 h, at 37C, 150 rpm with: 4, 12, and 20 g/mL amikacin or 10, 15, and 30 mM GSH or a combination of 30 mM GSH + 4 g/mL amikacin whereas, controls were treated with 1 PBS
However, you may have a preferred time of day when its best for you to manage the side effects
For in vitro samples both the media and lysate were normalized to the lysate protein
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