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Investigators using KLOW in multi-week protocols must maintain cold chain integrity or risk administering degraded, ineffective compound while assuming therapeutic dosing
After incubation with the primary antibody, the cells were washed with PBST and stained with secondary antibodies (Goat Anti-Rabbit IgG H&L Alexa Fluor 488, Abcam, ab150077
Resveratrol prevents beta-cell dedifferentiation in nonhuman primates given a high-fat/high-sugar diet
Aortic tissue LOXL2 activity assay Aortas from young ( 18 months old) WT and LOXL2+/ littermates were extracted and cleaned free of connective tissue in Krebs-HEPES buffer (NaCl 135 mM, KCl 5 mM, MgSO 4 1 mM, K 2 HPO 4 0.4 mM, Glucose 5.5 mM, HEPES 20 mM, adjust the pH to 7.4, supplement with 1 mM CaCl 2 ) containing 0.25 g/mL fungizone (Gibco)
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