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Repeatedly opening and closing a vial introduces fresh oxygen, providing the fuel for oxidative degradation
These effects have been confirmed in controlled studies, where trained cyclists riding in simulated altitude improved time-trial outcomes alongside reduced submaximal oxygen consumption (VO) after a single dose of beetroot
Furthermore, when exposed to high-altitude hypoxia, mouse hippocampal neurons exhibit decreased mitochondrial length, increased fragmentation of mitochondria, and decreased membrane potential and oxidative phosphorylation, resulting in increased mitochondrial fission and dysfunction (86)
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