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S5B), suggesting that catabolism liver-derived circulating GSH was not contributing to GSH levels in tissues
A 1 nM TGR was used in all cases
Target proteins were detected with an HRP-conjugated anti-rabbit or anti-mouse IgG secondary antibody (Cell Signaling Technology), followed by a chemiluminescent method (Millipore, Burlington, MA)
4.4 Sulfur-containing amino acid metabolism and renal protection 4.4.1 Metabolic fate of methionine and cysteine Sulfur-containing amino acids (methionine and cysteine) play critical roles in maintaining redox balance and producing protective gaseous signaling molecules (140)
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