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Description

DNA extraction was performed, and 500 ng of DNA was subjected to bisulfite conversion using the EZ DNA Methylation Kit from Zymo Research, following the manufacturers protocol

Subsequently, the ejaculates of Holstein bulls were pooled, split into four equal groups, and diluted with a Tris extender buffer supplemented with basic extender (negative control group, NC group), 2 g/ml SeNPs (SeNPs group), 4 mM GSH (GSH group), and 4 mM GSH plus 2 g/ml SeNPs (GSH + SeNPs group)