We usually test before starting treatment, then after 1-3 months
Never reuse needles or syringes, and follow local regulations for disposing of medical sharps
Applications include dehydrogenase and oxidoreductase assays monitored at 340 nm, sirtuin deacylase activity assays, PARP and ADP-ribosyltransferase reactions, CD38 and NADase kinetics, NAD+/NADH ratio measurement as a readout of cytosolic and mitochondrial redox state, and cellular ageing research where tissue NAD+ decline has been attributed largely to increased consumption by CD38 (Camacho-Pereira et al., Cell Metabolism, 2016) and quantified by stable-isotope flux analysis (Liu et al., Cell Metabolism, 2018)
Typical REGEN protocols run 4-8 weeks for acute injuries, 8-12 weeks for chronic conditions, and 4-6 weeks for athletic recovery and maintenance