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It is the predominant intracellular non-protein thiol in mammalian tissues, present at concentrations typically ranging from 110 mM in the cytosol, with particularly high concentrations in the liver (up to 10 mM), which is the primary site of GSH synthesis and export
The cysteine thiol group (-SH) confers redox activity, enabling reversible oxidation-reduction cycling between reduced (GSH) and oxidized (GSSG) states
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