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Spectral editing was accomplished by refocusing GSH J-evolution during every other acquisition (ON), using a 43-ms Gaussian pulse centered at the cysteinyl -CH resonance of GSH at 4.56 p.p.m

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Potential identities were then added to an inhouse structure library including.mol files from Chemspider (Royal Society of Chemistry, London, United Kingdom) and then targeted on the initial raw data according to the following parameters: a 5 ppm mass tolerance, a 2 mDa mass tolerance for predicted structure fragments, a 5% CCS deviation, and multiple adducts (+H, + Na, +K, + Li, +NH4) within 5 ppm and 0.1 minutes

These observations reinforce the need for well-designed randomised controlled trials (RCTs) to validate the findings and expand the evidence base