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Following a 1 h incubation with the appropriate secondary antibody, Clarity Western ECL Substrate was applied to the membrane and bands were visualized using a Biorad Chemidoc MP to first pixel saturation and densitometry performed using Image Lab (Biorad, Hercules, CA)
After amplification, concentrations were determined based on the proportion of nonfluorescent partitions through the Poisson distribution
p = 0.002
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