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It is also worth seeking advice if you follow a vegan or vegetarian diet, take medications that may affect B12 absorption, or have a digestive condition that may increase your risk of deficiency
A 10 g cell pellet was resuspended in 30 ml buffer B (25 mM Tris-HCl, pH 7.5, 10% glycerol, 0.01% Igepal CA-630, 2 mM 2-Mercaptoethanol) containing 500 mM KCl and a cocktail of protease inhibitors (PIs) (5 g/ml each of aprotinin, chymostatin, leupeptin, and pepstatin A) and lysed by sonication (QSonica Q700) at an amplitude of 40 for two min with 20 s pulses
A 2020 chemo support study used daily 1000mg doses for cisplatin protection
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