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It is primarily manifested by increased intracellular iron levels and enhanced lipid peroxidation, ultimately leading to cell death characterized by mitochondrial shrinkage, loss of cristae, and disruption of membrane integrity ( The Gut Microbiota, as a vital component of the intestinal microenvironment, plays a pivotal role in maintaining intestinal homeostasis, modulating host immune responses, participating in metabolic regulation, and promoting nutrient absorption

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The anagen phase ratio, calculated as the number of hair follicles in the anagen phase out of all follicles, was measured on days 3, 6, and 9 of culture to evaluate the maintenance rate of the growth phase