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Beta-hCG-producing Renal Cell Carcinoma [PMID: 26582059] Laboratory literature summary: Beta-hCG-producing Renal Cell Carcinoma
The GST construct (Amersham Biosciences) in pGEX-4T-3 vector was purified by manual glutathione-affinity chromatography using Glutathione Sepharose 4 fast flow beads (#17-5132-01, GE Healthcare) and eluted with 10 mM reduced glutathione (#11514, SRL), 150 mM NaCl and 2 mM DTT
Subsequently, ChIP assays were performed using anti-p-CREB (cell signaling technology), anti-CBP (cell signaling technology), anti-H3K4me3 (cell signaling technology), or anti-H2AK119ub antibodies (Abcam, United Kingdom) and a negative control comprising normal rabbit IgG (Santa Cruz Biotechnology, Inc., United States)
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