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TSPCs (5,000 cells/cm 2 ) were treated in triplicate with vehicle as the N group, 125, 250, 500, 1000, or 2000 M NAC in 96-well plates in F12 complete medium in a 5% CO 2 incubator at 37 C for 6 and 24 h
Room temperature storage reduces the safe usage window to approximately 14 days
Additionally, high-protein diets, which are commonly recommended while on tirzepatide to preserve muscle mass , generate more urea as a metabolic byproduct
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