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Materials and methods Animals All work involving rats was approved by the Institutional Animal Care and Use Committee of the Sahlgrenska Academy at the University of Gothenburg, Sweden and was performed in adherence with the Guide for the Care and Use of Laboratory Animals

On the basis of the ability of GLP-1RA/tesaglitazar to induce neuronal activation in the hypothalamus, and recent data indicating that PPAR might affect systemic energy metabolism via hypothalamic neurocircuitries 5,6 , we next assessed the response of the hypothalamic proteome of DIO mice 10 h after single peripheral (s.c.) drug treatment using liquid chromatographymass spectrometry (LCMS)

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