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mTOR-dependent phosphorylation controls TFEB nuclear export
In brief, HUVECs were lysed in the Co-IP buffer containing 20 mM Tris-HCl (pH7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 0.5% protease inhibitor cocktail on ice for 20 min
Storage and Handling Peptides are sensitive molecules that can degrade if not stored and handled properly
Homocysteine can be converted back to methionine in a reaction catalyzed by vitamin B 12 -dependent methionine synthase, which requires 5-methyltetrahydrofolate (5-meTHF) as a methyl donor
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