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Western blot analysis Protein lysates were obtained by homogenizing tissues or cell pellets in sample buffer containing 62.5 mM Tris-HCl (pH 6.8), 4% SDS, 200 mM dithiothreitol (DTT), 10% glycerol, and 0.001% bromophenol blue using a tissue to buffer ratio of 1:10 (w/v), followed by boiling
Worlds Poult Sci J 76(3):459471 Nabi F, Arain MA, Rajput N, Alagawany M, Soomro J, Umer M, Soomro F, Wang Z, Ye R, Liu J (2020) Health benefits of carotenoids and potential application in poultry industry: a review
Biomarkers of selenium status
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