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Remarkably, significant conformational changes of ECL1 upon receptor activation were observed relative to their inactive conformations: (i) in the inactive GCGR, ECL1 forms a -hairpin conformation and further compacts -sheet with the stalk region
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Accordingly, in INS-1 832/3 and MIN6B1 cells, accumulation of cAMP (with a low concentration of 3-isobutyl-1-methylxanthine (IBMX) to prevent cAMP degradation) was greater with exendin-phe1 than with exendin-4 and exendin-asp3 (Fig
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