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Split-APEX and split-BioID were introduced earlier, but split-APEX requires the addition of H 2 O 2 and ferrous heme, limiting its in vivo use, while split-BioID has very low activity

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However, if the defenses are too weak, then infections cannot be handled or body cells can proliferate uncontrolled and grow to form tumors, which can become life threatening. In vivo, T-cell-specific ablation of murine Gclc prevented autoimmune disease but blocked antiviral defense, the Immunity article noted

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