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This minor change not only affects the protonation of the chromophore leading to pronounced stabilization of the anionic form of the chromophore (Elsliger et al., E from 280 mV for roGFP2 to 238 mV for roGFP2-iL is lower than the shift for roGFP1-iL which at 229 mV is 62 mV less negative than its parent roGFP1

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Furukawa, T., Meydani, S

Required Equipment and Reagents Lyophilized BPC-157 vial (typically 5mg or 10mg) Bacteriostatic Water (0.9% benzyl alcohol) or Sterile Normal Saline Alcohol prep pads (70% Isopropyl Alcohol) Sterile syringes (typically U-100 insulin syringes or graduated tuberculin syringes) A clean, draft-free workspace (ideally a laminar flow hood) Step-by-Step Mixing Instructions First, prepare the workspace by sanitizing all surfaces with isopropyl alcohol