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Dual-label FISH for Glp1r and Chat The spinal cord sections were hybridized with the cocktail of the digoxigenin-labeled antisense Glp1r riboprobe, and a 2326 bases long fluorescein-labeled antisense Chat riboprobe transcribed from a cDNA template corresponding to the full-length rat Chat mRNA (Genbank # XM_032919318.1) (kind gift from Sylvie Berrard, Universit de Paris, NeuroDiderot, Inserm, Paris, France)
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In maintenance only two or three cells remain
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