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Western blotting Cells were harvested and suspended in Total Lysis Buffer (0.125 M Tris-HCl pH 6.8, 4% SDS and 20% glycerol)
In another rat study, brain infarction was significantly lower in animals which were treated intranasally with KND (a DSIP analog) at a dose of 300 mcg/kg, compared to controls [28]
Experimental results showed a modulation interaction of DSIP with endogenous opioid-peptidergic systems and exogenous intracerebrally or systemically administered morphine and amphetamine
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