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The drug consists of two peptides that, when combined, are more effective in reducing weight: one reduces the sensation of hunger while the other regulates the energy balance and the accumulation of fat in the body
For sample cleanup, 1 L of Exo-SAP-IT (ThermoFisher) was added to 2.5 L of PCR product and incubated at 37 C for 15 min followed by a 15 min incubation at 80 C to inactivate the enzyme
The antioxidant properties help in detoxifying it
The following numbers of cells were plated in six-well plates: U2OS: 2 10 6 cells per well, SUP-B15: 6 10 5 cells per well and THP-1: 1 10 6 cells per well
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