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The genes encoding Cezanne (residues 53-446, E287K E288K), UbcH5c (residues 1-147), and E3C (residues 693-1083) were previously cloned into the pCold-SUMO expression vector 53
Unlike anabolic steroids that primarily increase the size of existing muscle fibers (hypertrophy), IGF-1 LR3 may also promote hyperplasia the formation of new muscle cells a potential advantage for long-term growth
Briefly, 2 10 4 yeast cells/well were used to infect A-431 cells in 96-well plates at 37 C and 5% CO 2
J.EdwardsR.GoldsbroughP
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