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The existing methods for phenotypic selection of genetically modified mammalian cells are mainly based on three types of phenotypic markers: an exogenous drug resistance gene that permits screening for positive cells, a fluorescent protein, such as GFP, that allows isolating positive cells by FACS (fluorescence-activated cell sorting) (Chen et al., 1999), or a membrane protein that enables antibody-based immunomagnetic selection of positive cells (Plouffe et al., 2015)
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