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If it feels thicker, stickier, or creates bubbles that do not dissipate normally, the formulation may have changed
In obesity states, hepatic BCKDH is excessively phosphorylated, inhibiting its activity (22)
To some extent, yes
Sections were washed with TBS (3 10 min), mounted on SuperFrost glass slides (MenzelGlaser) using 0.5% Gelatine (SigmaAldrich) + 0.05% Chromalum (BDH Chemicals Ltd) dissolved in dH 2 O, dried at room temperature for 20 min, rehydrated in dH 2 O for 15 min, and dehydrated in a graded series of ethanol solutions (2 min in 96%, 5 min in 99%)
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