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Pemvidutide (ALT-801) is another unimolecular once weekly GLP-1/glucagon agonist undergoing a phase 2 trial as obesity treatment, following a phase 1 trial demonstrating up to 10.3% WL at 12 weeks [73]
Appropriate PPE may include gloves, laboratory coats, and eye protection where required by laboratory safety procedures
siRNA knockdown and plasmid overexpression For suspension cells, 1 10^6 cells were washed with OPTI-MEM (Gibco, USA) to remove serum, resuspended in OPTI-MEM containing 50 nM siRNA (RiboBio, China) or 3 g/L plasmid (GeneRulor, China), and then transferred to an electrode cuvette
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