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Western blot analysis and Coomassie Brilliant Blue (CBB) staining Samples were boiled in sample loading buffer at 100 C for 10 min, separated by SDS-PAGE, and transferred onto NC membranes for blotting

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Broad Connectivity Map analysis has shown that GHK-Cu can suppress genes associated with metastasis, tissue destruction, and inflammation while activating genes associated with stem cell maintenance and DNA repair [3]